Coding

Part:BBa_K936023:Design

Designed by: Pui Yan Ho   Group: iGEM12_UC_Davis   (2012-10-02)


Glycolaldehyde Reductase (aerobic)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 633
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
    Illegal AgeI site found at 1084
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SDM (site directed mutagenesis) performed on native glycolaldehyde reductase to create the aerobic form of the enzyme.


Source

e. coli MG1655

References